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KMID : 0881720130280020124
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Journal of Food Hygiene and Safety
2013 Volume.28 No. 2 p.124 ~ p.129
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Development of PCR Method for Rapid Detection of Allergic Materials in Foods
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Park Yong-Chjun
Kim Mi-Ra
Shin Joon-Ho
Kim Kyu-Heon
Lee Jae-Hwang
Cho Tae-Yong
Lee Hwa-Jung
Han Sang-Bae
Lee Sang-Jae
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Abstract
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The method for detection foods containing allergenic materials by PCR was developed in this study. To detect allergenic raw material from processed food, species specific primer which up to 200bp for PCR product were designed or selected from advanced research. As target materials, 14 items were selected (12 target materials for allergen in Korea, 2 target materials for allergen in foreign countries). The amplicon size for eggs, milk, buckwheat, peanuts, beans, wheat, mackerel, crab, shrimp, pork, peach, tomato, almond, and sesame were confirmed 281, 131, 138, 120, 118, 127, 211, 174, 231, 138, 174, 132, 103, and 220bp, respectively. And any non-specific bands were not detected among each others. Detection method for allergenic material developed in this study could be used to investigate inaccurate goods for allergen labeling or non-intentional contaminant during processed foods manufacturing. In addition, the system will be usefully to detection accurate allergenic raw materials of export for other countries.
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KEYWORD
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Allergen, PCR (Polymerase Chain Reaction), Species-specific primer
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